Fluorescein-12-dCTP

Biorbyt

Fluorescein-12-dCTP

16

Incorporation into DNA/cDNA by: PCR with Taqpolymerasein-house data; Nick Translation with DNAse I/DNA Polymerase Iin-house data. Spectroscopic Propertie: lambdaexc 492 nm, lambdaem 517 nm, epsilon 83.0 L mmol-1 cm-1 (Tris-HCl pH 7.5).

Research Use Only

1.0-1.1 mM

Fluorescein-12-dCTP is recommended for direct enzymatic labeling of DNA/cDNA e.g. by PCR and Nick Translation. It is incorporated as substitute for its natural counterpart dCTP. The resulting Dye-labeled DNA/cDNA probes are ideally suited for fluorescence hybridization applications such as FISH or microarray-based gene expression profiling. Optimal substrate properties and thus labeling efficiency is ensured by an optimized linker attached to the C5 position of cytidine. Recommended Fluorescein-12-dCTP/dCTP ratio for PCR and Nick Translation: 30-50% Fluorescein-12-dCTP-/70-50% dCTP.

-20°C

12352200