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Mouse liver lysates probed with Rabbit Anti-FMO5 Polyclonal Antibody (bs-13187R) at 1:300 overnight in 4˚C. Followed by conjugation to the secondary antibody (bs-0295G-HRP) at 1:5000 90min in 37˚C.
Mouse liver lysates probed with Rabbit Anti-FMO5 Polyclonal Antibody (bs-13187R) at 1:300 overnight in 4˚C. Followed by conjugation to the secondary antibody (bs-0295G-HRP) at 1:5000 90min in 37˚C.
Mouse liver lysates probed with Rabbit Anti-FMO5 Polyclonal Antibody (bs-13187R) at 1:300 overnight in 4˚C. Followed by conjugation to the secondary antibody (bs-0295G-HRP) at 1:5000 90min in 37˚C.

FMO5 Polyclonal Antibody

BS-13187R
Bioss
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityBovine, Canine, Human, Mouse, Porcine, Rat
TargetFMO5
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Overview

  • Supplier
    Bioss
  • Product Name
    FMO5 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID2330
  • Target name
    FMO5
  • Target description
    flavin containing dimethylaniline monoxygenase 5
  • Target synonyms
    hBVMO1, flavin-containing monooxygenase 5, FMO 5, NADPH oxidase, NAPDH oxidase, baeyer-Villiger monooxygenase 1, dimethylaniline monooxygenase [N-oxide-forming] 5, dimethylaniline oxidase 5, flavin containing monooxygenase 5, hepatic flavin-containing monooxygenase 5
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP49326
  • Protein Name
    Flavin-containing monooxygenase 5
  • Reactivity
    Bovine, Canine, Human, Mouse, Porcine, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

Datasheet

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Figure 1. Western blot analysis of FMO5 using anti-FMO5 antibody (PA2108). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FMO5 antigen affinity purified polyclonal antibody (Catalog # PA2108) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FMO5 at approximately 60 kDa. The expected band size for FMO5 is at 60 kDa.
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