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The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using CSB-PA916967(FMR1 Antibody) at dilution 1/30, on the right is treated with fusion protein. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using CSB-PA916967(FMR1 Antibody) at dilution 1/30, on the right is treated with fusion protein. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using CSB-PA916967(FMR1 Antibody) at dilution 1/30, on the right is treated with fusion protein. (Original magnification: x200)

FMR1 Antibody

CSB-PA916967
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetFMR1
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Overview

  • Supplier
    Cusabio
  • Product Name
    FMR1 Antibody
  • Delivery Days Customer
    20
  • Applications
    ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID2332
  • Target name
    FMR1
  • Target description
    FMRP translational regulator 1
  • Target synonyms
    FMRP; fragile X mental retardation 1; fragile X mental retardation protein 1; FRAXA; POF; POF1; synaptic functional regulator FMR1; truncated FMRP
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ06787
  • Protein Name
    Synaptic functional regulator FMR1
  • Scientific Description
    The protein encoded by this gene binds RNA and is associated with polysomes. The encoded protein may be involved in mRNA trafficking from the nucleus to the cytoplasm. A trinucleotide repeat (CGG) in the 5 UTR is normally found at 6-53 copies, but an expansion to 55-230 repeats is the cause of fragile X syndrome. Expansion of the trinucleotide repeat may also cause one form of premature ovarian failure (POF1). Multiple alternatively spliced transcript variants that encode different protein isoforms and which are located in different cellular locations have been described for this gene.
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of FMRP/FMR1 using anti-FMRP/FMR1 antibody (PB9703). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat liver tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FMRP/FMR1 antigen affinity purified polyclonal antibody (Catalog # PB9703) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FMRP/FMR1 at approximately 71-73 kDa. The expected band size for FMRP/FMR1 is at 71 kDa.
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