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ProductsBack/FOS Like Antigen 1 (FOSL1) Polyclonal Antibody

FOS Like Antigen 1 (FOSL1) Polyclonal Antibody

CAU21803
Biomatik
ApplicationsImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
TargetFOSL1
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Overview

  • Supplier
    Biomatik
  • Product Name
    FOS Like Antigen 1 (FOSL1) Polyclon
  • Delivery Days Customer
    12
  • Applications
    ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Applications Supplier
    WB; IHC; ICC; IP.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID8061
  • Target name
    FOSL1
  • Target description
    FOS like 1, AP-1 transcription factor subunit
  • Target synonyms
    FOS like 1, AP-1 trancription factor subunit; FOS-like antigen-1; fos-related antigen 1; FRA; FRA1; fra-1
  • Host
    Rabbit
  • Protein IDP15407
  • Protein Name
    Fos-related antigen 1
  • Scientific Description
    The FOS Like Antigen 1 (FOSL1) Polyclonal Antibody (Species: Human) has been validated for the following applications: WB, IHC, ICC, IP.
  • Reactivity Supplier
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of FRA1 using anti-FRA1 antibody (A03927-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A375 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FRA1 antigen affinity purified polyclonal antibody (Catalog # A03927-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FRA1 at approximately 29KD. The expected band size for FRA1 is at 29KD.
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Lane 1: Hela cell lysates; Lane 2: U87MG cell lysates probed with Phospho-FRA1 (Ser265) Polyclonal Antibody, Unconjugated (bs-3158R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
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