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Western Blot analysis of Jurkat cells using FoxK1 Polyclonal Antibody
Western Blot analysis of Jurkat cells using FoxK1 Polyclonal Antibody
Western Blot analysis of Jurkat cells using FoxK1 Polyclonal Antibody

FOXK1 Antibody

CSB-PA008225
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetFOXK1
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Overview

  • Supplier
    Cusabio
  • Product Name
    FOXK1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID221937
  • Target name
    FOXK1
  • Target description
    forkhead box K1
  • Target synonyms
    FOXK1L, forkhead box protein K1, MNF, myocyte nuclear factor
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP85037
  • Protein Name
    Forkhead box protein K1
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of FOXK1 using anti-FOXK1 antibody (A07015-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human U20S whole cell lysates, Lane 4: human A431 whole cell lysates, Lane 5: human Hela whole cell lysates, Lane 6: human T-47D whole cell lysates, Lane 7: human CACO-2 whole cell lysates, Lane 8: human HL-60 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FOXK1 antigen affinity purified polyclonal antibody (Catalog # A07015-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FOXK1 at approximately 97 kDa. The expected band size for FOXK1 is at 97 kDa.
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