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FOXL2 antibody

10R-1545
Biosynth
Product group Antibodies
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Overview

  • Supplier
    Biosynth
  • Product Name
    FOXL2 antibody
  • Delivery Days Customer
    7
  • Certification
    Research Use Only
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of FOXL2 using anti-FOXL2 antibody (A01185-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: rat ovary tissue lysates, Lane 3: mouse ovary tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FOXL2 antigen affinity purified polyclonal antibody (Catalog # A01185-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FOXL2 at approximately 50 kDa. The expected band size for FOXL2 is at 50 kDa.
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Immunohistochemistry analysis in human ovary and liver tissues using AMAb91808 antibody. Corresponding FOXL2 RNA-seq data are presented for the same tissues.
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FOXL2 antibody [C3], C-term detects FOXL2 protein by western blot analysis. Mouse tissue extracts (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with FOXL2 antibody [C3], C-term (GTX100200) diluted at 1:500.
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