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Gel: 10%SDS-PAGE, Lysate: 40 ug, Lane 1-2: Mouse brain tissue, Mouse heart tissue, Primary antibody: CSB-PA981648(FOXL2 Antibody) at dilution 1/750, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 30 seconds
Gel: 10%SDS-PAGE, Lysate: 40 ug, Lane 1-2: Mouse brain tissue, Mouse heart tissue, Primary antibody: CSB-PA981648(FOXL2 Antibody) at dilution 1/750, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 30 seconds
Gel: 10%SDS-PAGE, Lysate: 40 ug, Lane 1-2: Mouse brain tissue, Mouse heart tissue, Primary antibody: CSB-PA981648(FOXL2 Antibody) at dilution 1/750, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 30 seconds

FOXL2 Antibody

CSB-PA981648
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetFOXL2
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Overview

  • Supplier
    Cusabio
  • Product Name
    FOXL2 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID668
  • Target name
    FOXL2
  • Target description
    forkhead box L2
  • Target synonyms
    BPES; BPES1; forkhead box protein L2; forkhead transcription factor FOXL2; PFRK; PINTO; POF3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP58012
  • Protein Name
    Forkhead box protein L2
  • Scientific Description
    This gene encodes a forkhead transcription factor. The protein contains a fork-head DNA-binding domain and may play a role in ovarian development and function. Mutations in this gene are a cause of blepharophimosis syndrome and premature ovarian failure 3. Critical factor essential for ovary differentiation and maintenance, and repression of the genetic program for somatic testis determination. Prevents trans-differentiation of ovary to testis through transcriptional repression of the Sertoli cell-promoting gene SOX9. Activates SIRT1 transcription under cellular stress conditions. Activates transcription of OSR2.
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of FOXL2 using anti-FOXL2 antibody (A01185-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: rat ovary tissue lysates, Lane 3: mouse ovary tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FOXL2 antigen affinity purified polyclonal antibody (Catalog # A01185-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FOXL2 at approximately 50 kDa. The expected band size for FOXL2 is at 50 kDa.
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