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FOXP2 Rabbit pAb

A5677
ABclonal
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetFOXP2
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Overview

  • Supplier
    ABclonal
  • Product Name
    FOXP2 Rabbit pAb
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID93986
  • Target name
    FOXP2
  • Target description
    forkhead box P2
  • Target synonyms
    CAGH44, SPCH1, TNRC10, forkhead box protein P2, CAG repeat protein 44, forkhead/winged-helix transcription factor, trinucleotide repeat containing 10, trinucleotide repeat-containing gene 10 protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO15409
  • Protein Name
    Forkhead box protein P2
  • Reactivity
    Human, Mouse, Rat
  • Reactivity Supplier Note
    Human,Mouse,Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of FOXP2 using anti-FOXP2 antibody (A01329-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysate,Lane 2: mouse brain tissue lysate,Lane 3: human SK-OV-3 whole Cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FOXP2 antigen affinity purified polyclonal antibody (Catalog # A01329-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FOXP2 at approximately 90KD. The expected band size for FOXP2 is at 80KD.
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Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with FOXP2 antibody (GTX135285) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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TargetFOXP2
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