Non-transfected (–) and transfected (+) boiled and unboiled 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Frizzled 4 antibody [HL2824] (GTX640095) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
![Boiled and unboiled various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Frizzled 4 antibody [HL2824] (GTX640095) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](https://www.genetex.com/upload/website/prouct_img/normal/GTX640095/GTX640095_T-45355_20240419_WB_24042400_308.webp "Boiled and unboiled various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Frizzled 4 antibody [HL2824] (GTX640095) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![Unboiled various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Frizzled 4 antibody [HL2824] (GTX640095) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](https://www.genetex.com/upload/website/prouct_img/normal/GTX640095/GTX640095_45439_20240621_WB_TPM_watermark_24062501_395.webp "Unboiled various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Frizzled 4 antibody [HL2824] (GTX640095) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.")
![Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Frizzled 4 antibody [HL2824] (GTX640095) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.](https://www.genetex.com/upload/website/prouct_img/normal/GTX640095/GTX640095_45439_20240628_WB_Z_24070822_141.webp "Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Frizzled 4 antibody [HL2824] (GTX640095) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.")
![Unboiled non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Frizzled 4 antibody [HL2824] (GTX640095) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX640095/GTX640095_45439_20250502_WB_multiple_B_25051420_126.webp "Unboiled non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Frizzled 4 antibody [HL2824] (GTX640095) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
Non-transfected (–) and transfected (+) boiled and unboiled 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Frizzled 4 antibody [HL2824] (GTX640095) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Frizzled 4 antibody [HL2824]
GTX640095
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Zebra Fish
TargetFZD4
Overview
- SupplierGeneTex
- Product NameFrizzled 4 antibody [HL2824]
- Delivery Days Customer7
- Application Supplier NoteWB: 1:500-1:10000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDHL2824
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID8322
- Target nameFZD4
- Target descriptionfrizzled class receptor 4
- Target synonymsCD344, EVR1, FEVR, FZD4S, Fz-4, Fz4, FzE4, GPCR, hFz4, frizzled-4, WNT receptor frizzled-4, frizzled 4, seven transmembrane spanning receptor, frizzled family receptor 4, frizzled homolog 4
- HostRabbit
- IsotypeIgG
- Protein IDQ9ULV1
- Protein NameFrizzled-4
- Scientific DescriptionThis gene is a member of the frizzled gene family. Members of this family encode seven-transmembrane domain proteins that are receptors for the Wingless type MMTV integration site family of signaling proteins. Most frizzled receptors are coupled to the beta-catenin canonical signaling pathway. This protein may play a role as a positive regulator of the Wingless type MMTV integration site signaling pathway. A transcript variant retaining intronic sequence and encoding a shorter isoform has been described, however, its expression is not supported by other experimental evidence. [provided by RefSeq, Jul 2008]
- ReactivityHuman, Zebra Fish
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
Datasheet
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