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ProductsBack/Frizzled 4 antibody [HL2824]

Frizzled 4 antibody [HL2824]

GTX640095
GeneTex
ApplicationsWestern Blot
DownloadDatasheet
Product group Antibodies
TargetFZD4
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Overview

  • Supplier
    GeneTex
  • Product Name
    Frizzled 4 antibody [HL2824]
  • Delivery Days Customer
    7
  • Application Supplier Note
    WB: 1:500-1:10000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    HL2824
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID8322
  • Target name
    FZD4
  • Target description
    frizzled class receptor 4
  • Target synonyms
    CD344; EVR1; FEVR; frizzled 4, seven transmembrane spanning receptor; frizzled family receptor 4; frizzled homolog 4; frizzled-4; Fz4; Fz-4; FZD4S; FzE4; GPCR; hFz4; WNT receptor frizzled-4
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9ULV1
  • Protein Name
    Frizzled-4
  • Scientific Description
    This gene is a member of the frizzled gene family. Members of this family encode seven-transmembrane domain proteins that are receptors for the Wingless type MMTV integration site family of signaling proteins. Most frizzled receptors are coupled to the beta-catenin canonical signaling pathway. This protein may play a role as a positive regulator of the Wingless type MMTV integration site signaling pathway. A transcript variant retaining intronic sequence and encoding a shorter isoform has been described, however, its expression is not supported by other experimental evidence. [provided by RefSeq, Jul 2008]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Frizzled 4 using anti-Frizzled 4 antibody (A02191-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human MCF-7 cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Frizzled 4 antigen affinity purified polyclonal antibody (Catalog # A02191-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Frizzled 4 at approximately 65KD. The expected band size for Frizzled 4 is at 60KD.
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