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IHC-P analysis of human lung carcinoma tissue using GTX51938 FRMD6 antibody. Dilution : 1:200
IHC-P analysis of human lung carcinoma tissue using GTX51938 FRMD6 antibody. Dilution : 1:200
IHC-P analysis of human lung carcinoma tissue using GTX51938 FRMD6 antibody. Dilution : 1:200

FRMD6 antibody

GTX51938
GeneTex
ApplicationsImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetFRMD6
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Overview

  • Supplier
    GeneTex
  • Product Name
    FRMD6 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    IHC-P: 1:50-400. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID122786
  • Target name
    FRMD6
  • Target description
    FERM domain containing 6
  • Target synonyms
    C14orf31, EX1, Willin, c14_5320, FERM domain-containing protein 6, 4.1 ezrin radixin moesin (FERM)-containing protein, expanded homolog
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96NE9
  • Protein Name
    FERM domain-containing protein 6
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of FRMD6 using anti-FRMD6 antibody (A08826-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human PANC-1 whole cell lysates, Lane 4: human CACO-2 whole cell lysates, Lane 5: rat PC-12 whole cell lysates, Lane 6: mouse Neuro-2a whole cell lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FRMD6 antigen affinity purified polyclonal antibody (Catalog # A08826-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FRMD6 at approximately 72KD. The expected band size for FRMD6 is at 72KD.
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