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ProductsBack/FST / Follistatin Antibody (aa35-283)

FST / Follistatin Antibody (aa35-283)

LS-C314491
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetFST
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    FST / Follistatin Antibody (aa35-283)
  • Delivery Days Customer
    23
  • Application Supplier Note
    ELISA 1:100-1:5000; ICC 1:50-500; WB 1:50-400;
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:100 - 1:400) ELISA 1:100-1:5000; ICC 1:50-500; WB 1:50-400;
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.36 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID10468
  • Target name
    FST
  • Target description
    follistatin
  • Target synonyms
    activin-binding protein; follistatin; FS
  • Host
    Rabbit
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Follistatin using anti-Follistatin antibody (A00972-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse kidney tissue lysates, Lane 2: human MCF-7 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Follistatin antigen affinity purified polyclonal antibody (Catalog # A00972-1) at 0.5 ug/mL overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Follistatin at approximately 40KD. The expected band size for Follistatin is at 38KD.
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