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FTL / Ferritin Light Chain Antibody

LS-C125573
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetFTL
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    FTL / Ferritin Light Chain Antibody
  • Delivery Days Customer
    14
  • Application Supplier Note
    Suitable for use in ELISA and Western Blot. ELISA: 1:100-1:500. Western Blot: 1:1000-1:20000.
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    ELISA (1:100 - 1:500), WB (1:1000 - 1:20000) Suitable for use in ELISA and Western Blot. ELISA: 1:100-1:500. Western Blot: 1:1000-1:20000.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID2512
  • Target name
    FTL
  • Target description
    ferritin light chain
  • Target synonyms
    epididymis secretory sperm binding protein; ferritin L subunit; ferritin L-chain; ferritin light chain; ferritin light polypeptide-like 3; ferritin, light polypeptide; LFTD; NBIA3
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Ferritin Light Chain using anti-Ferritin Light Chain antibody (M01956-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human THP-1 whole cell lysates, Lane 4: human SiHa whole cell lysates, Lane 5: rat liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Ferritin Light Chain antigen affinity purified monoclonal antibody (Catalog # M01956-1) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Ferritin Light Chain at approximately 20 kDa. The expected band size for Ferritin Light Chain is at 20 kDa.
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