Fura-2, Pentaammonium Salt

Biotium

Fura-2, pentaammonium salt

9

Other Application

Research Use Only

Fura-2, pentaammonium salt is a ratiometric fluorescent dye that binds to free intracellular calcium. It is membrane-impermeant and may be loaded into cells via microinjection or scrape loading. A major use of Fura-2 is in drug discovery, for measuring intracellular calcium mobilization after activation of G protein-coupled receptors (GPCRs) and calcium channels. Upon calcium binding, the fluorescent excitation maximum of the indicator undergoes a blue shift from 363 nm (Ca2-free) to 335 nm (Ca2-saturated), while the fluorescence emission maximum is relatively unchanged at ~510 nm. Light yellow solid soluble in DMSO Store at 4°C and protect from light, especially in solution Kd: 145 nM (22°C in pH 7.2 buffer) lambda Ex/lambdaEm (no Ca2) = 363/512 nm; lambda Ex/lambda Em (high Ca2) = 335/505 nm epsilon (363 nm, no Ca2) = 27,000 M-1cm-1; epsilon (335 nm, high Ca2) = 35,000 M-1cm-1 C29H37N8O14 MW: 721

2°C to 8°C

41116134