Fura-FF AM

Biorbyt

Fura-FF AM [348079-12-9]

10

348079-12-9

Research Use Only

98.00%

C43H43F2N3O24

1023.811

Fura-FF AM is a cell-permeable acetoxymethyl ester of the fluorescence calcium indicator fura-FF (potassium salt). Upon entering cells, it is hydrolyzed by intracellular esterases to produce fura-FF, a difluorinated derivative of the calcium indicator fura-2. Unlike fura-2, fura-FF has negligible magnesium sensitivity, reducing interference from this cation, and a higher calcium dissociation constant (Kd(calcium) = 6 microM for fura-FF and 0.14 microM for fura-2). The spectral properties of fura-FF are similar to those of fura-2, with fura-FF displaying excitation/emission spectra of 365/514 nm in the absence of calcium and shifting to 339/507 nm in the presence of high calcium concentrations. Low affinity calcium dyes, including fura-FF, are preferred for studying compartments with high calcium concentrations, such as mitochondria, or in cell systems with relatively low calcium buffering capacities, such as neuronal dendrites and spines.

CC(=O)OCOC(=O)CN(CC(=O)OCOC(C)=O)c1cc2oc(cc2cc1OCCOc1c(F)c(F)ccc1N(CC(=O)OCOC(C)=O)CC(=O)OCOC(C)=O)-c1ncc(o1)C(=O)OCOC(C)=O

-20°C

12352200