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The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using CSB-PA752513(FUT1 Antibody) at dilution 1/30, on the right is treated with synthetic peptide. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using CSB-PA752513(FUT1 Antibody) at dilution 1/30, on the right is treated with synthetic peptide. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using CSB-PA752513(FUT1 Antibody) at dilution 1/30, on the right is treated with synthetic peptide. (Original magnification: x200)

FUT1 Antibody

CSB-PA752513
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetFUT1
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Overview

  • Supplier
    Cusabio
  • Product Name
    FUT1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID2523
  • Target name
    FUT1
  • Target description
    fucosyltransferase 1 (H blood group)
  • Target synonyms
    H, HH, HSC, galactoside alpha-(1,2)-fucosyltransferase 1, GDP-L-fucose:beta-D-galactoside 2-alpha-L-fucosyltransferase 1, H antigen, H glycosyltransferase, H glycosytransferase, a-1, 2 fucosyltransferase (H antigen), alpha(1,2) fucosyltransferase 1, alpha(1,2)FT 1, blood group H alpha 2-fucosyltransferase, galactoside 2-L-fucosyltransferase enzyme, galactoside 2-alpha-L-fucosyltransferase, nonfunctional fucosyltransferase 1, type 1 galactoside alpha-(1,2)-fucosyltransferase FUT1, type 2 galactoside alpha-(1,2)-fucosyltransferase FUT1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP19526
  • Protein Name
    Galactoside alpha-(1,2)-fucosyltransferase 1
  • Scientific Description
    The protein encoded by this gene is a Golgi stack membrane protein that is involved in the creation of a precursor of the H antigen, which is required for the final step in the soluble A and B antigen synthesis pathway. This gene is one of two encoding the galactoside 2-L-fucosyltransferase enzyme. Mutations in this gene are a cause of the H-Bombay blood group.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of FUT1 using anti-FUT1 antibody (PB9593). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Kidney Tissue Lysate at 50ug, Lane 2: SW620 Whole Cell Lysate at 40ug, Lane 3: A549 Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FUT1 antigen affinity purified polyclonal antibody (Catalog # PB9593) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FUT1 at approximately 50 kDa. The expected band size for FUT1 is at 50 kDa.
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