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FXR1 Antibody

CAB5942
Assay Genie
TargetFXR1
Product group Antibodies
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Overview

  • Supplier
    Assay Genie
  • Product Name
    FXR1 Rabbit pAb
  • Delivery Days Customer
    9
  • Applications Supplier
    WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID8087
  • Target name
    FXR1
  • Target description
    FMR1 autosomal homolog 1
  • Target synonyms
    CMYO9A, CMYO9B, CMYP9A, CMYP9B, FXR1P, MYOPMIL, MYORIBF, RNA-binding protein FXR1, FMR1 autosomal protein-like protein 1
  • Host
    Rabbit
  • Protein IDP51114
  • Protein Name
    Fragile X mental retardation syndrome-related protein 1
  • Shelf life instruction
    12 months
  • Source
    Rabbit
  • Reactivity Supplier
    Human,Mouse,Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Western Blot Positive WB detected in: HEK293 whole cell lysate, Hela whole cell lysate, NIH/3T3 whole cell lysate All lanes: FXR1 antibody at 3ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 70, 61, 60 kDa Observed band size: 70 kDa
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Figure 1. Western blot analysis of FXR1 using anti-FXR1 antibody (A03308-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: monkey skeletal muscle tissue lysates, Lane 4: monkey heart tissue lysates, Lane 5: rat heart tissue lysates, Lane 6: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FXR1 antigen affinity purified polyclonal antibody (Catalog # A03308-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FXR1 at approximately 80 kDa. The expected band size for FXR1 is at 80 kDa.
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