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Western Blot analysis of Hela cells using Frizzled-1 Polyclonal Antibody
Western Blot analysis of Hela cells using Frizzled-1 Polyclonal Antibody
Western Blot analysis of Hela cells using Frizzled-1 Polyclonal Antibody

FZD1 Antibody

CSB-PA002579
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetFZD1
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Overview

  • Supplier
    Cusabio
  • Product Name
    FZD1 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID8321
  • Target name
    FZD1
  • Target description
    frizzled class receptor 1
  • Target synonyms
    frizzled-1, Wnt receptor, frizzled 1, seven transmembrane spanning receptor, frizzled family receptor 1, frizzled homolog 1, frizzled, Drosophila, homolog of, 1, fz-1, fzE1, hFz1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UP38
  • Protein Name
    Frizzled-1
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Frizzled homolog 1 using anti-Frizzled homolog 1 antibody (PB9594). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: 22RV1 Whole Cell Lysate at 40ug, Lane 2: 293T Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Frizzled homolog 1 antigen affinity purified polyclonal antibody (Catalog # PB9594) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Frizzled homolog 1 at approximately 71 kDa. The expected band size for Frizzled homolog 1 is at 71 kDa.
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