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Paraformaldehyde-fixed, paraffin embedded rat kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Rabbit Anti-FZD3/Frizzled 3 Polyclonal Antibody, Unconjugated (bs-13239R) at 1:200 overnight at 4°C, followed by the secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded rat kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Rabbit Anti-FZD3/Frizzled 3 Polyclonal Antibody, Unconjugated (bs-13239R) at 1:200 overnight at 4°C, followed by the secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded rat kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Rabbit Anti-FZD3/Frizzled 3 Polyclonal Antibody, Unconjugated (bs-13239R) at 1:200 overnight at 4°C, followed by the secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml) was used to stain the cell nuclei.

FZD3 Polyclonal Antibody

BS-13239R
Bioss
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetFZD3
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Overview

  • Supplier
    Bioss
  • Product Name
    FZD3 Polyclonal Antibody
  • Delivery Days Customer
    7
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF()
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID7976
  • Target name
    FZD3
  • Target description
    frizzled class receptor 3
  • Target synonyms
    Fz-3, frizzled-3, frizzled 3, seven transmembrane spanning receptor, frizzled family receptor 3, frizzled homolog 3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9NPG1
  • Protein Name
    Frizzled-3
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Su L, Zhang Z, Gan L, et al. Deregulation of the planar cell polarity genes CELSR3 and FZD3 in Hirschsprung disease. Exp Mol Pathol. 2016,101(2):241-248. doi: 10.1016/j.yexmp.2016.09.003
    Read this paper

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Figure 1. Western blot analysis of FZD3 using anti-FZD3 antibody (A04680-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human SK-OV-3 cell lysates, Lane 2: human Jurkat cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FZD3 antigen affinity purified polyclonal antibody (Catalog # A04680-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FZD3 at approximately 76KD. The expected band size for FZD3 is at 76KD.
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