![Immunoprecipitation of GABPA protein from 293T whole cell extracts using 5 μg of GABPA antibody [N2C2], Internal (GTX102572). Western blot analysis was performed using GABPA antibody [N2C2], Internal (GTX102572). EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent. Immunoprecipitation of GABPA protein from 293T whole cell extracts using 5 μg of GABPA antibody [N2C2], Internal (GTX102572). Western blot analysis was performed using GABPA antibody [N2C2], Internal (GTX102572). EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.](https://www.genetex.com/upload/website/prouct_img/normal/GTX102572/GTX102572_42004_20151016_IP_w_23060100_165.webp)
Immunoprecipitation of GABPA protein from 293T whole cell extracts using 5 μg of GABPA antibody [N2C2], Internal (GTX102572). Western blot analysis was performed using GABPA antibody [N2C2], Internal (GTX102572). EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
GABPA antibody [N2C2], Internal
GTX102572
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetGABPA
Overview
- SupplierGeneTex
- Product NameGABPA antibody [N2C2], Internal
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration0.33 mg/ml
- ConjugateUnconjugated
- Gene ID2551
- Target nameGABPA
- Target descriptionGA binding protein transcription factor subunit alpha
- Target synonymsE4TF1-60, E4TF1A, NFT2, NRF2, NRF2A, RCH04A07, GA-binding protein alpha chain, GA binding protein transcription factor alpha subunit 60kDa, GABP subunit alpha, human nuclear respiratory factor-2 subunit alpha, nuclear respiratory factor 2 alpha subunit, nuclear respiratory factor 2 subunit alpha, transcription factor E4TF1-60
- HostRabbit
- IsotypeIgG
- Protein IDQ06546
- Protein NameGA-binding protein alpha chain
- Scientific DescriptionThis gene encodes one of three GA-binding protein transcription factor subunits which functions as a DNA-binding subunit. Since this subunit shares identity with a subunit encoding the nuclear respiratory factor 2 gene, it is likely involved in activation of cytochrome oxidase expression and nuclear control of mitochondrial function. This subunit also shares identity with a subunit constituting the transcription factor E4TF1, responsible for expression of the adenovirus E4 gene. Because of its chromosomal localization and ability to form heterodimers with other polypeptides, this gene may play a role in the Down Syndrome phenotype. [provided by RefSeq]
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
![GABPA antibody [N2C2], Internal detects GABPA protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: GABPA protein stained by GABPA antibody [N2C2], Internal (GTX102572) diluted at 1:1000. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Blue: Hoechst 33342 staining. Scale bar = 10 μm. GABPA antibody [N2C2], Internal detects GABPA protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: GABPA protein stained by GABPA antibody [N2C2], Internal (GTX102572) diluted at 1:1000. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Blue: Hoechst 33342 staining. Scale bar = 10 μm.](https://www.genetex.com/upload/website/prouct_img/normal/GTX102572/GTX102572_42004_20150525_IFA_w_23060100_806.webp)
![GABPA antibody [N2C2], Internal detects GABPA protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded rat spleen. GABPA stained by GABPA antibody [N2C2], Internal (GTX102572) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min GABPA antibody [N2C2], Internal detects GABPA protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded rat spleen. GABPA stained by GABPA antibody [N2C2], Internal (GTX102572) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX102572/GTX102572_44048_20210507_IHC-P_R_w_23060100_616.webp)
![GABPA antibody [N2C2], Internal detects GABPA protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse lymph node. GABPA stained by GABPA antibody [N2C2], Internal (GTX102572) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min GABPA antibody [N2C2], Internal detects GABPA protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse lymph node. GABPA stained by GABPA antibody [N2C2], Internal (GTX102572) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX102572/GTX102572_44048_20210507_IHC-P_M_w_23060100_308.webp)
![Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GABPA antibody [N2C2], Internal (GTX102572) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GABPA antibody [N2C2], Internal (GTX102572) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX102572/GTX102572_44048_20200904_WB_23061320_938.webp)






