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Western Blot Positive WB detected in: HepG2 whole cell lysate All lanes: GABRB3 antibody at 2.7microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 55, 47, 46 kDa Observed band size: 55 kDa
Western Blot Positive WB detected in: HepG2 whole cell lysate All lanes: GABRB3 antibody at 2.7microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 55, 47, 46 kDa Observed band size: 55 kDa
Western Blot Positive WB detected in: HepG2 whole cell lysate All lanes: GABRB3 antibody at 2.7microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 55, 47, 46 kDa Observed band size: 55 kDa

GABRB3 Antibody

CSB-PA009148LA01HU
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetGABRB3
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Overview

  • Supplier
    Cusabio
  • Product Name
    GABRB3 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID2562
  • Target name
    GABRB3
  • Target description
    gamma-aminobutyric acid type A receptor subunit beta3
  • Target synonyms
    DEE43; ECA5; EIEE43; GABAA receptor beta-3 subunit; GABA-alpha receptor beta-2 subunit; gamma-aminobutyric acid (GABA) A receptor, beta 3; gamma-aminobutyric acid A receptor beta 3; gamma-aminobutyric acid receptor subunit beta-3; gamma-aminobutyric acid type A receptor beta3 subunit
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP28472
  • Protein Name
    Gamma-aminobutyric acid receptor subunit beta-3
  • Scientific Description
    Component of the heteropentameric receptor for GABA, the major inhibitory neurotransmitter in the vertebrate brain. Functions also as histamine receptor and mediates cellular responses to histamine. Functions as receptor for diazepines and various anesthetics, such as pentobarbital; these are bound at a separate allosteric effector binding site. Functions as ligand-gated chloride channel.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of GABRB3 using anti-GABRB3 antibody (PB9595). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Brain Tissue Lysate at 50ug, Lane 2: Mouse Brain Tissue Lysate at 50ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GABRB3 antigen affinity purified polyclonal antibody (Catalog # PB9595) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GABRB3 at approximately 54 kDa. The expected band size for GABRB3 is at 54 kDa.
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