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Formalin-fixed and paraffin embedded rat brain labeled with Anti-GADD153 Polyclonal Antibody, Unconjugated (bs-1361R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded rat brain labeled with Anti-GADD153 Polyclonal Antibody, Unconjugated (bs-1361R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded rat brain labeled with Anti-GADD153 Polyclonal Antibody, Unconjugated (bs-1361R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

DDIT3 Polyclonal Antibody

BS-1361R
Bioss
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetDDIT3
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Overview

  • Supplier
    Bioss
  • Product Name
    DDIT3 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID1649
  • Target name
    DDIT3
  • Target description
    DNA damage inducible transcript 3
  • Target synonyms
    AltDDIT3, C/EBPzeta, CEBPZ, CHOP, CHOP-10, CHOP10, GADD153, DNA damage-inducible transcript 3 protein, C/EBP zeta, CCAAT/enhancer-binding protein homologous protein, alternative DDIT3 protein, c/EBP-homologous protein 10, growth arrest and DNA damage-inducible protein GADD153
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP35638
  • Protein Name
    DNA damage-inducible transcript 3 protein
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

References

  • C/EBP Homologous Protein (CHOP) Activates Macrophages and Promotes Liver Fibrosis in Schistosoma japonicum-Infected Mice. Duan M et al., 2019, J Immunol Res
    Read this paper
  • KLF15-activating Twist2 ameliorated hepatic steatosis by inhibiting inflammation and improving mitochondrial dysfunction via NF-kappaB-FGF21 or SREBP1c-FGF21 pathway. Zhou L et al., 2019 Dec, FASEB J
    Read this paper
  • Acetaldehyde Induces Neurotoxicity In Vitro via Oxidative Stress- and Ca2+ Imbalance-Mediated Endoplasmic Reticulum Stress. Cui J et al., 2019, Oxid Med Cell Longev
    Read this paper
  • Role of the Death Receptor and Endoplasmic Reticulum Stress Signaling Pathways in Polyphyllin I-Regulated Apoptosis of Human Hepatocellular Carcinoma HepG2 Cells. Luo Q et al., 2018, Biomed Res Int
    Read this paper
  • Radioprotective effect of Hohenbuehelia serotina polysaccharides through mediation of ER apoptosis pathway in vivo. Wang L et al., 2019 Apr 15, Int J Biol Macromol
    Read this paper
  • Chaihulonggumulitang Shows Psycho-cardiology Therapeutic Effects on Acute Myocardial Infarction by Enhancing Bone Marrow Mesenchymal Stem Cells Mobilization. Wang C et al., 2018 Feb 27, Sci Rep
    Read this paper
  • Matrine inhibits the progression of prostate cancer by promoting expression of GADD45B. Huang H et al., 2018 Apr, Prostate
    Read this paper
  • Silver nanoparticles induce SH-SY5Y cell apoptosis via endoplasmic reticulum- and mitochondrial pathways that lengthen endoplasmic reticulum-mitochondria contact sites and alter inositol-3-phosphate receptor function. Li L et al., 2018 Mar 15, Toxicol Lett
    Read this paper
  • SelK is a novel ER stress-regulated protein and protects HepG2 cells from ER stress agent-induced apoptosis. Du S et al., 2010 Oct 15, Arch Biochem Biophys
    Read this paper

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Figure 1. Western blot analysis of DDIT3 using anti-DDIT3 antibody (A00311-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human U-87MG whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human THP-1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DDIT3 antigen affinity purified polyclonal antibody (Catalog # A00311-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DDIT3 at approximately 29KD. The expected band size for DDIT3 is at 29KD.
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