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Western Blot analysis of Jurkat cells using GADD 45gamma Polyclonal Antibody
Western Blot analysis of Jurkat cells using GADD 45gamma Polyclonal Antibody
Western Blot analysis of Jurkat cells using GADD 45gamma Polyclonal Antibody

GADD45G Antibody

CSB-PA002624
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetGADD45G
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Overview

  • Supplier
    Cusabio
  • Product Name
    GADD45G Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10912
  • Target name
    GADD45G
  • Target description
    growth arrest and DNA damage inducible gamma
  • Target synonyms
    CR6, DDIT2, GADD45gamma, GRP17, growth arrest and DNA damage-inducible protein GADD45 gamma, DDIT-2, DNA damage-inducible transcript 2 protein, GADD45-gamma, cytokine-responsive protein CR6, gadd-related protein, 17 kD
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO95257
  • Protein Name
    Growth arrest and DNA damage-inducible protein GADD45 gamma
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of GADD45G using anti-GADD45G antibody (A04681-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: rat heart tissue lysates, Lane 3: rat testis tissue lysates, Lane 4: rat skeletal muscle tissue lysates, Lane 5: mouse brain tissue lysates, Lane 6: mouse heart tissue lysates, Lane 7: mouse testis tissue lysates, Lane 8: mouse skeletal muscle tissue lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GADD45G antigen affinity purified polyclonal antibody (Catalog # A04681-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GADD45G at approximately 19KD. The expected band size for GADD45G is at 17KD.
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