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GAL4 Antibody (YA3377)

HY-P83632
MedChem Express
TargetLGALS4
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    GAL4 Antibody (YA3377)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID3960
  • Target name
    LGALS4
  • Target description
    galectin 4
  • Target synonyms
    antigen NY-CO-27; GAL4; gal-4; galectin-4; L-36 lactose-binding protein; L36LBP; lactose-binding lectin 4; lectin, galactoside-binding, soluble, 4
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    GAL4 Antibody (YA3377) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA3377), targeting GAL4, with a predicted molecular weight of 36 kDa (observed band size: 36 kDa). GAL4 Antibody (YA3377) can be used for WB, IHC-P experiment in human, rat background.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of GAL4 using anti-GAL4 antibody (PB9952). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: SW620 whole cell lysates, Lane 2: COLO320 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GAL4 antigen affinity purified polyclonal antibody (Catalog # PB9952) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GAL4 at approximately 39 kDa. The expected band size for GAL4 is at 36 kDa.
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