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Galectin 1 (GAL1) Polyclonal Antibody

CAU28105
Biomatik
ApplicationsImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityMouse, Rat
TargetLgals1
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Overview

  • Supplier
    Biomatik
  • Product Name
    Galectin 1 (GAL1) Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Applications Supplier
    WB; IHC; ICC; IP.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID56646
  • Target name
    Lgals1
  • Target description
    galectin 1
  • Target synonyms
    galectin-1, 14 kDa lectin, RL 14.5, S-Lac lectin 1, beta-galactoside-binding lectin L-14-I, gal-1, galaptin, lactose-binding lectin 1, lectin, galactose binding, soluble 1, lectin, galactoside-binding, soluble, 1
  • Host
    Rabbit
  • Protein IDP11762
  • Protein Name
    Galectin-1
  • Scientific Description
    The Galectin 1 (GAL1) Polyclonal Antibody (Species: Rat) has been validated for the following applications: WB, IHC, ICC, IP.
  • Reactivity
    Mouse, Rat
  • Reactivity Supplier
    Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Galectin 1 using anti-Galectin 1 antibody (A00470-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat gaster tissue lysates, Lane 2: rat ovary tissue lysates, Lane 3: rat testis tissue lysates, Lane 4: mouse kidney tissue lysates, Lane 5: human Hela whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Galectin 1 antigen affinity purified polyclonal antibody (Catalog # A00470-1) at 0.5 ug/mL overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Galectin 1 at approximately 15KD. The expected band size for Galectin 1 is at 15KD.
Product group Antibodies
Boster Bio
Anti-Galectin 1/Lgals1 Antibody Picoband(r)A00470-1-CARRIER-FREE
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetLgals1
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