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Immunohistochemistry of paraffin-embedded human gastric cancer using CSB-PA814224LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human gastric cancer using CSB-PA814224LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human gastric cancer using CSB-PA814224LA01HU at dilution of 1:100

GALNT12 Antibody

CSB-PA814224LA01HU
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetGALNT12
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Overview

  • Supplier
    Cusabio
  • Product Name
    GALNT12 Antibody
  • Delivery Days Customer
    20
  • Applications
    ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID79695
  • Target name
    GALNT12
  • Target description
    polypeptide N-acetylgalactosaminyltransferase 12
  • Target synonyms
    colorectal cancer, susceptibility to 1; CRCS1; GalNAc-T12; polypeptide GalNAc transferase 12; polypeptide N-acetylgalactosaminyltransferase 12; pp-GaNTase 12; protein-UDP acetylgalactosaminyltransferase 12; UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 12; UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 12 (GalNAc-T12)
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8IXK2
  • Protein Name
    Polypeptide N-acetylgalactosaminyltransferase 12
  • Scientific Description
    Catalyzes the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor. Has activity toward non-glycosylated peptides such as Muc5AC, Muc1a and EA2, and no detectable activity with Muc2 and Muc7. Displays enzymatic activity toward the Gal-NAc-Muc5AC glycopeptide, but no detectable activity to mono-GalNAc-glycosylated Muc1a, Muc2, Muc7 and EA2. May play an important role in the initial step of mucin-type oligosaccharide biosynthesis in digestive organs.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of GALNT12 using anti-GALNT12 antibody (A09946). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human Hacat whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human A431 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GALNT12 antigen affinity purified polyclonal antibody (Catalog # A09946) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GALNT12 at approximately 67 kDa. The expected band size for GALNT12 is at 67 kDa.
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