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Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA822698LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA822698LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA822698LA01HU at dilution of 1:100

GALNT15 Antibody

CSB-PA822698LA01HU
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetGALNT15
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Overview

  • Supplier
    Cusabio
  • Product Name
    GALNT15 Antibody
  • Delivery Days Customer
    20
  • Applications
    ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID117248
  • Target name
    GALNT15
  • Target description
    polypeptide N-acetylgalactosaminyltransferase 15
  • Target synonyms
    GALNACT15, GALNT13, GALNT7, GALNTL2, PIH5, pp-GalNAc-T15, polypeptide N-acetylgalactosaminyltransferase 15, UDP-GalNAc transferase T15, UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase-like protein 2, UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 15, UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 7, UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase-like 2, galNAc-T-like protein 2, polypeptide GalNAc transferase 15, polypeptide GalNAc transferase-like protein 2, polypeptide N-acetylgalactosaminyltransferase 13, polypeptide N-acetylgalactosaminyltransferase-like protein 2, pp-GaNTase-like protein 2, pregnancy-induced hypertension syndrome-related protein 5, protein-UDP acetylgalactosaminyltransferase-like protein 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8N3T1
  • Protein Name
    Polypeptide N-acetylgalactosaminyltransferase 15
  • Scientific Description
    Catalyzes the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor. Although it displays a much weaker activity toward all substrates tested compared to GALNT2, it is able to transfer up to seven GalNAc residues to the Muc5AC peptide, suggesting that it can fill vicinal Thr/Ser residues in cooperation with other GALNT proteins. Prefers Muc1a as substrate.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of GALNT15 using anti-GALNT15 antibody (A11598-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: rat spleen tissue lysates, Lane 3: rat ovary tissue lysates, Lane 4: mouse spleen tissue lysates, Lane 5: mouse ovary tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GALNT15 antigen affinity purified polyclonal antibody (Catalog # A11598-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GALNT15 at approximately 73 kDa. The expected band size for GALNT15 is at 73 kDa.
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