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GALNT2 Antibody

LS-C346137
LifeSpan BioSciences
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
Product group Antibodies
TargetGALNT2
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    GALNT2 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 30kDa/64kDa, while the observed MW by Western blot was 65kDa.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
  • Applications Supplier
    IF (1:50 - 1:100), IHC (1:50 - 1:200), WB (1:500 - 1:2000) The predicted MW is 30kDa/64kDa, while the observed MW by Western blot was 65kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID2590
  • Target name
    GALNT2
  • Target description
    polypeptide N-acetylgalactosaminyltransferase 2
  • Target synonyms
    CDG2T, GalNAc-T2, polypeptide N-acetylgalactosaminyltransferase 2, UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 2, UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 2 (GalNAc-T2), polypeptide GalNAc transferase 2, pp-GaNTase 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of GALNT2 using anti-GALNT2 antibody (A04458-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human MOLT-4 whole cell lysates, Lane 4: rat liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GALNT2 antigen affinity purified polyclonal antibody (Catalog # A04458-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GALNT2 at approximately 65 kDa. The expected band size for GALNT2 is at 65 kDa.
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