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GALT Antibody (YA2925, Rabbit)

HY-P83180
MedChem Express
TargetGALT
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    GALT Antibody (YA2925, Rabbit)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID2592
  • Target name
    GALT
  • Target description
    galactose-1-phosphate uridylyltransferase
  • Target synonyms
    gal-1-P uridylyltransferase; galactose-1-phosphate uridyl transferase; galactose-1-phosphate uridylyltransferase; UDP-glucose--hexose-1-phosphate uridylyltransferase
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    GALT Antibody (YA2925) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA2925), targeting GALT, with a predicted molecular weight of 43 kDa (observed band size: 43 kDa). GALT Antibody (YA2925) can be used for WB, IP experiment in human, mouse, rat background.
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of GALT using anti-GALT antibody (A01460-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HepG2 cell lysates, Lane 2: human MCF-7 cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GALT antigen affinity purified polyclonal antibody (Catalog # A01460-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GALT at approximately 48KD. The expected band size for GALT is at 43KD.
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