gamma-(2-Azidoethyl)-ATP

Jena Bioscience

gamma-(2-Azidoethyl)-ATP

5

Research Use Only

Lee et al.[1] reported a non-radioactive version of in vitro phosphorylation were gamma-[2-Azidoethyl]-ATP (compound 8[1]) has been successfully used instead of gamma-32P-modified ATP to phosphorylate GST-tagged recombinant p27kip1 with protein kinase cdk2. The phosphorylated, azide-modified protein substrate can subsequently be labeled with Alkynes of biotin or fluorescent dyes via Cu(I)- catalyzed Click-Chemistry or DBCO-containing biotin or fluorescent dyes via Cu(I)-free Click-Chemistry. Presolski et al.[2] and Hong et al.[3] provide a general protocol for Cu(I)-catalyzed click chemistry reactions that may be used as a starting point for the set up and optimization of individual assays.

-20°C

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