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gamma Catenin Antibody (YA420, Rabbit)

HY-P80683
MedChem Express
TargetJUP
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    gamma Catenin Antibody (YA420, Rabbit)
  • Delivery Days Customer
    10
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID3728
  • Target name
    JUP
  • Target description
    junction plakoglobin
  • Target synonyms
    catenin (cadherin-associated protein), gamma 80kDa; CTNNG; desmoplakin III; desmoplakin-3; desmosomal protein 3; DP3; DPIII; junction plakoglobin; PDGB; PG; PKGB
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    gamma Catenin Antibody (YA420) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to gamma Catenin.
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of gamma Catenin using anti-gamma Catenin antibody (PB9185). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human T47D whole cell lysates, Lane 4: rat heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-gamma Catenin antigen affinity purified polyclonal antibody (Catalog # PB9185) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for gamma Catenin at approximately 82 kDa. The expected band size for gamma Catenin is at 82 kDa.
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