gamma-[(Propargyl)-imido]-ATP

Jena Bioscience

gamma-[(Propargyl)-imido]-ATP

5

Research Use Only

≥95%

C13H19N6O12P3 (free acid)

544.24 g/mol (free acid)

Lee et al.[1] reported a non-radioactive version of in vitro phosphorylation were gamma-[(Propargyl)-imido]-ATP (compound 1[1]) has been successfully used instead of gamma-32P-modified ATP to phosphorylate GST-tagged recombinant p27kip1 with protein kinase cdk2. The phosphorylated, alkyne-modified protein substrate can subsequently be labeled with azides of biotin or fluorescent dyes via Cu(I)- catalyzed Click-Chemistry. Presolski et al.[2] and Hong et al.[3] provide a general protocol for Cu(I)-catalyzed click chemistry reactions that may be used as a starting point for the set up and optimization of individual assays.

c1(c2c(ncn1)n(cn2)[C@H]1[C@H](O)[C@H](O)[C@H](O1)COP(=O)(O)OP(=O)(O)OP(=O)(NCC#C)O)N

-20°C

12352200