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ProductsBack/gamma Synuclein (SNCG) Rabbit Monoclonal Antibody [Clone ID: SAIC-31A-10]
Flow cytometry using the Anti-SNCG antibody SAIC-31A-10 (TA385904). Paraformaldehyde fixed U251 cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (TA385792; isotype control, black line) or the rabbit IgG version of SAIC-31A-10 (TA385904, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.
Flow cytometry using the Anti-SNCG antibody SAIC-31A-10 (TA385904). Paraformaldehyde fixed U251 cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (TA385792; isotype control, black line) or the rabbit IgG version of SAIC-31A-10 (TA385904, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.
Flow cytometry using the Anti-SNCG antibody SAIC-31A-10 (TA385904). Paraformaldehyde fixed U251 cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (TA385792; isotype control, black line) or the rabbit IgG version of SAIC-31A-10 (TA385904, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

gamma Synuclein (SNCG) Rabbit Monoclonal Antibody [Clone ID: SAIC-31A-10]

TA385904
OriGene
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetSNCG
200 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    gamma Synuclein (SNCG) Rabbit Monoclonal Antibody [Clone ID: SAIC-31A-10]
  • Delivery Days Customer
    14
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    SAIC-31A-10
  • Gene ID6623
  • Target name
    SNCG
  • Target description
    synuclein gamma
  • Target synonyms
    BCSG1, SR, gamma-synuclein, -synuclein, E pound-synuclein, breast cancer-specific gene 1 protein, persyn, synoretin, synuclein, gamma (breast cancer-specific protein 1)
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    Recombinant Anti-SNCG (Clone SAIC-31A-10)
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of gamma Synuclein/SNCG using anti-gamma Synuclein/SNCG antibody (A03523-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human RT4 whole cell lysates, Lane 3: human Hacat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-gamma Synuclein/SNCG antigen affinity purified polyclonal antibody (Catalog # A03523-4) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for gamma Synuclein/SNCG at approximately 17 kDa. The expected band size for gamma Synuclein/SNCG is at 13 kDa.
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