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Western Blot analysis of LOVO cells using Glucosidase IIalpha Polyclonal Antibody
Western Blot analysis of LOVO cells using Glucosidase IIalpha Polyclonal Antibody
Western Blot analysis of LOVO cells using Glucosidase IIalpha Polyclonal Antibody

GANAB Antibody

CSB-PA008333
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetGANAB
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Overview

  • Supplier
    Cusabio
  • Product Name
    GANAB Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID23193
  • Target name
    GANAB
  • Target description
    glucosidase II alpha subunit
  • Target synonyms
    G2AN, GIIA, GIIalpha, GLUII, PKD3, neutral alpha-glucosidase AB, alpha-glucosidase 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ14697
  • Protein Name
    Neutral alpha-glucosidase AB
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of GANAB using anti-GANAB antibody (A09668-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human THP-1 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human A431 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat kidney tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GANAB antigen affinity purified polyclonal antibody (Catalog # A09668-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GANAB at approximately 100 kDa. The expected band size for GANAB is at 107 kDa.
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