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Immunohistochemistry of paraffin-embedded human lung cancer using CSB-PA878917LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human lung cancer using CSB-PA878917LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human lung cancer using CSB-PA878917LA01HU at dilution of 1:100

GAR1 Antibody

CSB-PA878917LA01HU
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetGAR1
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Overview

  • Supplier
    Cusabio
  • Product Name
    GAR1 Antibody
  • Delivery Days Customer
    20
  • Applications
    ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID54433
  • Target name
    GAR1
  • Target description
    GAR1 ribonucleoprotein
  • Target synonyms
    NOLA1, H/ACA ribonucleoprotein complex subunit 1, GAR1 homolog, ribonucleoprotein, GAR1 ribonucleoprotein homolog, nucleolar protein family A member 1, nucleolar protein family A, member 1 (H/ACA small nucleolar RNPs), snoRNP protein GAR1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9NY12
  • Protein Name
    H/ACA ribonucleoprotein complex subunit 1
  • Scientific Description
    Required for ribosome biogenesis and telomere maintenance. Part of the H/ACA small nucleolar ribonucleoprotein (H/ACA snoRNP) complex, which catalyzes pseudouridylation of rRNA. This involves the isomerization of uridine such that the ribose is subsequently attached to C5, instead of the normal N1. Each rRNA can contain up to 100 pseudouridine (psi) residues, which may serve to stabilize the conformation of rRNAs. May also be required for correct processing or intranuclear trafficking of TERC, the RNA component of the telomerase reverse transcriptase (TERT) holoenzyme.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of NOLA1/GAR1 using anti-NOLA1/GAR1 antibody (A07049-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A375 whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human HL-60 whole cell lysates, Lane 4: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NOLA1/GAR1 antigen affinity purified polyclonal antibody (Catalog # A07049-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NOLA1/GAR1 at approximately 25 kDa. The expected band size for NOLA1/GAR1 is at 25 kDa.
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