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ProductsBack/GART antibody [N1N2], N-term
GART antibody [N1N2], N-term detects GART protein at cytoplasm on human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. GART antibody [N1N2], N-term (GTX114422) diluted at 1:500. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
GART antibody [N1N2], N-term detects GART protein at cytoplasm on human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. GART antibody [N1N2], N-term (GTX114422) diluted at 1:500. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
GART antibody [N1N2], N-term detects GART protein at cytoplasm on human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. GART antibody [N1N2], N-term (GTX114422) diluted at 1:500. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min

GART antibody [N1N2], N-term

GTX114422
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetGART
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Overview

  • Supplier
    GeneTex
  • Product Name
    GART antibody [N1N2], N-term
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:1000-1:10000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID2618
  • Target name
    GART
  • Target description
    phosphoribosylglycinamide formyltransferase, phosphoribosylglycinamide synthetase, phosphoribosylaminoimidazole synthetase
  • Target synonyms
    AIRS, GARS, GARTF, PAIS, PGFT, PRGS, trifunctional purine biosynthetic protein adenosine-3, GAR transformylase, GARS-AIRS-GART, glycinamide ribonucleotide formyltransferase, glycinamide ribonucleotide synthetase-aminoimidazole ribonucleotide synthetase-glycinamide ribonucleotide transformylase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP22102
  • Protein Name
    Trifunctional purine biosynthetic protein adenosine-3
  • Scientific Description
    The protein encoded by this gene is a trifunctional polypeptide. It has phosphoribosylglycinamide formyltransferase, phosphoribosylglycinamide synthetase, phosphoribosylaminoimidazole synthetase activity which is required for de novo purine biosynthesis. This enzyme is highly conserved in vertebrates. Alternative splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq]
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of GART using anti-GART antibody (A06061-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human THP-1 whole cell lysates, Lane 4: rat C6 whole cell lysates, Lane 5: mouse pancreas tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GART antigen affinity purified polyclonal antibody (Catalog # A06061-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GART at approximately 108 kDa. The expected band size for GART is at 108 kDa.
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