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ProductsBack/GART Recombinant Antibody
Lane 1: Hela Cells; Lane 2: 293T Cells; Lane 3: A431 Cells; Probed with GART (6A8) Monoclonal Antibody (bsm-52537R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Lane 1: Hela Cells; Lane 2: 293T Cells; Lane 3: A431 Cells; Probed with GART (6A8) Monoclonal Antibody (bsm-52537R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Lane 1: Hela Cells; Lane 2: 293T Cells; Lane 3: A431 Cells; Probed with GART (6A8) Monoclonal Antibody (bsm-52537R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.

GART Recombinant Antibody

BSM-52537R
Bioss
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetGART
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Overview

  • Supplier
    Bioss
  • Product Name
    GART Recombinant Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(WB=1:500), IHC-P(IHC-P=1:100-500), IHC-F(IHC-F=1:400-800), IF(IF=1:100-500), ICC/IF(ICC/IF=1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID2618
  • Target name
    GART
  • Target description
    phosphoribosylglycinamide formyltransferase, phosphoribosylglycinamide synthetase, phosphoribosylaminoimidazole synthetase
  • Target synonyms
    AIRS, GARS, GARTF, PAIS, PGFT, PRGS, trifunctional purine biosynthetic protein adenosine-3, GAR transformylase, GARS-AIRS-GART, glycinamide ribonucleotide formyltransferase, glycinamide ribonucleotide synthetase-aminoimidazole ribonucleotide synthetase-glycinamide ribonucleotide transformylase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP22102
  • Protein Name
    Trifunctional purine biosynthetic protein adenosine-3
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of GART using anti-GART antibody (A06061-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human THP-1 whole cell lysates, Lane 4: rat C6 whole cell lysates, Lane 5: mouse pancreas tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GART antigen affinity purified polyclonal antibody (Catalog # A06061-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GART at approximately 108 kDa. The expected band size for GART is at 108 kDa.
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