GAS Reporter (Luc)-HeLa Cell Line (IFNgamma/JAK/STAT1 Pathway)
79041
Product group (Cell) Culture
Overview
- SupplierBPS Bioscience
- Product NameGAS Reporter (Luc)-HeLa Cell Line (IFNgamma/JAK/STAT1 Pathway)
- Delivery Days Customer7
- CertificationResearch Use Only
- Scientific DescriptionThe GAS reporter (Luc)-HeLa cell line is designed to monitor the activity of interferon gamma-induced signal transduction pathways in cultured cells by measuring activated STAT1 homodimers. It contains a firefly luciferase gene driven by three copies of the interferon gamma-activated sites (GAS) located upstream of the minimal TATA promoter. IFNgamma first binds to a heterodimeric receptor consisting of two chains, IFNGR1 and IFNGR2, causing its dimerization and the activation of specific Janus family kinases (JAK1 and JAK2). Two STAT1 molecules associate with this ligand-activated receptor complex and are activated by phosphorylation to form active homodimer. The active STAT1 homodimers translocate to the nucleus where they bind interferon gamma-activated sites (GAS) in the promoter of IFNgamma inducible genes, including luciferase reporter gene.
- Storage InstructionLiquid Nitrogen
- UNSPSC12352207