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Western Blot Positive WB detected in: Jurkat whole cell lysate, Rat liver tissue All lanes: GAS2 antibody at 3.3microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 35, 16 kDa Observed band size: 35 kDa
Western Blot Positive WB detected in: Jurkat whole cell lysate, Rat liver tissue All lanes: GAS2 antibody at 3.3microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 35, 16 kDa Observed band size: 35 kDa
Western Blot Positive WB detected in: Jurkat whole cell lysate, Rat liver tissue All lanes: GAS2 antibody at 3.3microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 35, 16 kDa Observed band size: 35 kDa

GAS2 Antibody

CSB-PA009265LA01HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Rat
TargetGAS2
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Overview

  • Supplier
    Cusabio
  • Product Name
    GAS2 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID2620
  • Target name
    GAS2
  • Target description
    growth arrest specific 2
  • Target synonyms
    DFNB125, GAS-2, growth arrest-specific protein 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO43903
  • Protein Name
    Growth arrest-specific protein 2
  • Scientific Description
    May play a role in apoptosis by acting as a cell death substrate for caspases. Is cleaved during apoptosis and the cleaved form induces dramatic rearrangements of the actin cytoskeleton and potent changes in the shape of the affected cells. May be involved in the membrane ruffling process.
  • Reactivity
    Human, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of GAS2 using anti-GAS2 antibody (A08589-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: rat liver tissue lysates, Lane 4: mouse kidney tissue lysates, Lane 5: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GAS2 antigen affinity purified polyclonal antibody (Catalog # A08589-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GAS2 at approximately 35 kDa. The expected band size for GAS2 is at 35 kDa.
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