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Western Blot analysis of 293 cells using Gas6 Polyclonal Antibody
Western Blot analysis of 293 cells using Gas6 Polyclonal Antibody
Western Blot analysis of 293 cells using Gas6 Polyclonal Antibody

GAS6 Antibody

CSB-PA002641
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetGAS6
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Overview

  • Supplier
    Cusabio
  • Product Name
    GAS6 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID2621
  • Target name
    GAS6
  • Target description
    growth arrest specific 6
  • Target synonyms
    AXL receptor tyrosine kinase ligand; AXL stimulatory factor; AXLLG; AXSF; growth arrest-specific protein 6
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ14393
  • Protein Name
    Growth arrest-specific protein 6
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of GAS 6 using anti-GAS 6 antibody (A00608-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GAS 6 antigen affinity purified polyclonal antibody (Catalog # A00608-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GAS 6 at approximately 75KD. The expected band size for GAS 6 is at 79KD.
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