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Western Blot analysis of Human fetal brain and Mouse brain tissue using GAS7 Polyclonal Antibody at dilution of 1:625
Western Blot analysis of Human fetal brain and Mouse brain tissue using GAS7 Polyclonal Antibody at dilution of 1:625
Western Blot analysis of Human fetal brain and Mouse brain tissue using GAS7 Polyclonal Antibody at dilution of 1:625

GAS7 Polyclonal Antibody

E-AB-10340
Elabscience
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
TargetGAS7
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Overview

  • Supplier
    Elabscience
  • Product Name
    GAS7 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Applications Supplier
    ELISA WB IHC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.7 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID8522
  • Target name
    GAS7
  • Target description
    growth arrest specific 7
  • Target synonyms
    growth arrest-specific protein 7
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO60861
  • Protein Name
    Growth arrest-specific protein 7
  • Scientific Description
    Growth arrest-specific 7 is expressed primarily in terminally differentiated brain cells and predominantly in mature cerebellar Purkinje neurons. GAS7 plays a putative role in neuronal development. Several transcript variants encoding proteins which vary in the N-terminus have been described.
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of GAS7 using anti-GAS7 antibody (A06548-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human THP-1 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: rat testis tissue lysates, Lane 5: rat PC-12 whole cell lysates, Lane 6: mouse testis tissue lysates, Lane 7: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GAS7 antigen affinity purified polyclonal antibody (Catalog # A06548-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GAS7 at approximately 50 kDa. The expected band size for GAS7 is at 54 kDa.
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