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Western blot analysis of extracts of various cell lines using GAS7 Polyclonal Antibody at dilution of 1:3000.
Western blot analysis of extracts of various cell lines using GAS7 Polyclonal Antibody at dilution of 1:3000.
Western blot analysis of extracts of various cell lines using GAS7 Polyclonal Antibody at dilution of 1:3000.

GAS7 Polyclonal Antibody

E-AB-64949
Elabscience
Product group Antibodies
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Overview

  • Supplier
    Elabscience
  • Product Name
    GAS7 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Certification
    Research Use Only
  • Scientific Description
    Growth arrest-specific 7 is expressed primarily in terminally differentiated brain cells and predominantly in mature cerebellar Purkinje neurons. GAS7 plays a putative role in neuronal development. Several transcript variants encoding proteins which vary in the N-terminus have been described.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of GAS7 using anti-GAS7 antibody (A06548-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human THP-1 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: rat testis tissue lysates, Lane 5: rat PC-12 whole cell lysates, Lane 6: mouse testis tissue lysates, Lane 7: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GAS7 antigen affinity purified polyclonal antibody (Catalog # A06548-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GAS7 at approximately 50 kDa. The expected band size for GAS7 is at 54 kDa.
Product group Antibodies
Boster Bio
Anti-GAS7 Antibody Picoband(r)A06548-2-CARRIER-FREE
ApplicationsFlow Cytometry, Western Blot, ELISA
ReactivityHuman, Mouse, Rat
TargetGAS7
  • SizePrice
Product group Antibodies
RayBiotech
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ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
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Product group Antibodies
Biomatik
GAS7 Polyclonal AntibodyCAC14834
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityMouse, Rat
TargetGAS7
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Western Blot Positive WB detected in: Mouse brain tissue, Rat brain tissue All lanes: GAS7 antibody at 2.5ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 55, 48, 40 kDa Observed band size: 55 kDa
Product group Antibodies
Cusabio
GAS7 AntibodyCSB-PA009271LA01HU
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetGAS7
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Product group Antibodies
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TargetGAS7
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GAS7 AntibodyCAB14179
ReactivityHuman
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Product group Antibodies
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Product group Antibodies
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Sample (30 ug of whole cell lysate) A: A431 (GTX27909) 10% SDS PAGE GTX115101 diluted at 1:3000
Product group Antibodies
GeneTex
GAS7 antibody [N3C3]GTX115101
ApplicationsWestern Blot
ReactivityHuman
TargetGAS7
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