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GATA2 antibody

GTX113441
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
Product group Antibodies
TargetGATA2
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Overview

  • Supplier
    GeneTex
  • Product Name
    GATA2 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-Fr: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.38 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID2624
  • Target name
    GATA2
  • Target description
    GATA binding protein 2
  • Target synonyms
    DCML; endothelial transcription factor GATA-2; IMD21; MONOMAC; NFE1B
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP23769
  • Protein Name
    Endothelial transcription factor GATA-2
  • Scientific Description
    This gene encodes a member of the GATA family of zinc-finger transcription factors that are named for the consensus nucleotide sequence they bind in the promoter regions of target genes. The encoded protein plays an essential role in regulating transcription of genes involved in the development and proliferation of hematopoietic and endocrine cell lineages. Alternative splicing results in multiple transcript variants.
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • IL1beta Promotes TMPRSS2 Expression and SARS-CoV-2 Cell Entry Through the p38 MAPK-GATA2 Axis. Cioccarelli C et al., 2021, Front Immunol
    Read more
  • Cell Type- and Sex-Dependent Transcriptome Profiles of Rat Anterior Pituitary Cells. Fletcher PA et al., 2019, Front Endocrinol (Lausanne)
    Read more
  • Intrinsic Immunity Shapes Viral Resistance of Stem Cells. Wu X et al., 2018 Jan 25, Cell
    Read more

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Figure 1. Western blot analysis of GATA2 using anti-GATA2 antibody (A01415-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human HL-60 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GATA2 antigen affinity purified polyclonal antibody (Catalog # A01415-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GATA2 at approximately 51KD. The expected band size for GATA2 is at 51KD.
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