IHC-P analysis of human breast carcinoma tissue using GTX30600 GATA3 antibody [1A12-1D9]. Right : Primary antibody Left : Negative control without primary antibody Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min Dilution : 1:1000
![IHC-P analysis of mouse brain tissue using GTX30600 GATA3 antibody [1A12-1D9]. Right : Primary antibody Left : Negative control without primary antibody Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min Dilution : 1:1000](https://www.genetex.com/upload/website/prouct_img/normal/GTX30600/GTX30600_1333_IHC-P_w_23060722_377.webp "IHC-P analysis of mouse brain tissue using GTX30600 GATA3 antibody [1A12-1D9]. Right : Primary antibody Left : Negative control without primary antibody Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min Dilution : 1:1000")
![IHC-P analysis of rat brain tissue using GTX30600 GATA3 antibody [1A12-1D9]. Right : Primary antibody Left : Negative control without primary antibody Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min Dilution : 1:200](https://www.genetex.com/upload/website/prouct_img/normal/GTX30600/GTX30600_1334_IHC-P_w_23060722_756.webp "IHC-P analysis of rat brain tissue using GTX30600 GATA3 antibody [1A12-1D9]. Right : Primary antibody Left : Negative control without primary antibody Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min Dilution : 1:200")
![IP analysis of SH-SY5Y cell lysates using GTX30600 GATA3 antibody [1A12-1D9]. IP reaction : 5microl antibody / 250microg lysate Dilution : 1:1000](https://www.genetex.com/upload/website/prouct_img/normal/GTX30600/GTX30600_1449_IP_w_23060722_147.webp "IP analysis of SH-SY5Y cell lysates using GTX30600 GATA3 antibody [1A12-1D9]. IP reaction : 5microl antibody / 250microg lysate Dilution : 1:1000")
![WB analysis of 15 ug of SH-SY5Y whole cell lysate (WCL), SH-SY5Y nuclear extract (NE) using GTX30600 GATA3 antibody [1A12-1D9]. Dilution : 1:1000](https://www.genetex.com/upload/website/prouct_img/normal/GTX30600/GTX30600_1843_WB_w_23060722_383.webp "WB analysis of 15 ug of SH-SY5Y whole cell lysate (WCL), SH-SY5Y nuclear extract (NE) using GTX30600 GATA3 antibody [1A12-1D9]. Dilution : 1:1000")
![WB analysis of 15 ug of HEK293T cell lysate overexpressing GATA3-DDK (right lane) or empty vector control (left lane) using GTX30600 GATA3 antibody [1A12-1D9]. Dilution : 1:1000](https://www.genetex.com/upload/website/prouct_img/normal/GTX30600/GTX30600_2079_WB_w_23060722_672.webp "WB analysis of 15 ug of HEK293T cell lysate overexpressing GATA3-DDK (right lane) or empty vector control (left lane) using GTX30600 GATA3 antibody [1A12-1D9]. Dilution : 1:1000")
![ICC/IF analysis of MCF-7 cells using GTX30600 GATA3 antibody [1A12-1D9]. Cells were probed without (left) or with(right) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes Dilution : 1:50 for at least 1 hour at room temperature](https://www.genetex.com/upload/website/prouct_img/normal/GTX30600/GTX30600_727_ICC-IF_w_23060722_451.webp "ICC/IF analysis of MCF-7 cells using GTX30600 GATA3 antibody [1A12-1D9]. Cells were probed without (left) or with(right) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes Dilution : 1:50 for at least 1 hour at room temperature")
![ICC/IF analysis of SH-SY5Y cells using GTX30600 GATA3 antibody [1A12-1D9]. Cells were probed without (left) or with(right) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes Dilution : 1:50 for at least 1 hour at room temperature](https://www.genetex.com/upload/website/prouct_img/normal/GTX30600/GTX30600_729_ICC-IF_w_23060722_102.webp "ICC/IF analysis of SH-SY5Y cells using GTX30600 GATA3 antibody [1A12-1D9]. Cells were probed without (left) or with(right) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes Dilution : 1:50 for at least 1 hour at room temperature")
IHC-P analysis of human breast carcinoma tissue using GTX30600 GATA3 antibody [1A12-1D9]. Right : Primary antibody Left : Negative control without primary antibody Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min Dilution : 1:1000
GATA3 antibody [1A12-1D9]
GTX30600
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetGATA3
Overview
- SupplierGeneTex
- Product NameGATA3 antibody [1A12-1D9] - KO/KD, Orthogonal, Overexpression Validated
- Delivery Days Customer9
- Antibody SpecificityKnockdown/Knockout validation was supported by references (PMID:28805661)
- Application Supplier NoteWB: 1:1000. ICC/IF: 1:50-1:100. IHC-P: 1:200-1:2000. IP: 5microg. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID1A12-1D9
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID2625
- Target nameGATA3
- Target descriptionGATA binding protein 3
- Target synonymsGATA-binding factor 3; HDR; HDRS; trans-acting T-cell-specific transcription factor GATA-3
- HostMouse
- IsotypeIgG1
- Protein IDP23771
- Protein NameTrans-acting T-cell-specific transcription factor GATA-3
- Scientific DescriptionThis gene encodes a protein which belongs to the GATA family of transcription factors. The protein contains two GATA-type zinc fingers and is an important regulator of T-cell development and plays an important role in endothelial cell biology. Defects in this gene are the cause of hypoparathyroidism with sensorineural deafness and renal dysplasia. [provided by RefSeq, Nov 2009]
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- The FADS mouse: A novel mouse model of atopic keratoconjunctivitis. Nunomura S et al., 2021 Dec, J Allergy Clin ImmunolRead more
- The impact of the effectiveness of GATA3 as a prognostic factor in breast cancer. Fararjeh AS et al., 2018 Oct, Hum PatholRead more
- The FOXN3-NEAT1-SIN3A repressor complex promotes progression of hormonally responsive breast cancer. Li W et al., 2017 Sep 1, J Clin InvestRead more