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Western blot All lanes: Glycine amidinotransferase, mitochondrial antibody at 2ug/ml + Mouse kidney tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 49, 45 kDa Observed band size: 49 kDa
Western blot All lanes: Glycine amidinotransferase, mitochondrial antibody at 2ug/ml + Mouse kidney tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 49, 45 kDa Observed band size: 49 kDa
Western blot All lanes: Glycine amidinotransferase, mitochondrial antibody at 2ug/ml + Mouse kidney tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 49, 45 kDa Observed band size: 49 kDa

GATM Antibody

CSB-PA009284ESR1HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetGATM
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Overview

  • Supplier
    Cusabio
  • Product Name
    GATM Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID2628
  • Target name
    GATM
  • Target description
    glycine amidinotransferase
  • Target synonyms
    AGAT, AT, CCDS3, FRTS, FRTS1, RFS, glycine amidinotransferase, mitochondrial, glycine amidinotransferase (L-arginine:glycine amidinotransferase), testicular secretory protein Li 19, transamidinase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP50440
  • Protein Name
    Glycine amidinotransferase, mitochondrial
  • Scientific Description
    Catalyzes the biosynthesis of guanidinoacetate, the immediate precursor of creatine. Creatine plays a vital role in energy metabolism in muscle tissues. May play a role in embryonic and central nervous system development. May be involved in the response to heart failure by elevating local creatine synthesis.
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of GATM using anti-GATM antibody (A02754-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human AGS whole cell lysates, Lane 2: human HUH-7 whole cell lysates, Lane 3: rat kidney tissue lysates, Lane 4: rat HBZY-1 whole cell lysates, Lane 5: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GATM antigen affinity purified polyclonal antibody (Catalog # A02754-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GATM at approximately 48 kDa. The expected band size for GATM is at 48 kDa.
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