Wild-type (WT) and GBA knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GBA antibody [C1C3] (GTX101267) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
![MCF-7 whole cell and membrane extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GBA antibody [C1C3] (GTX101267) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX101267/GTX101267_43817_20200117_WB_Fraction_w_23060100_239.webp "MCF-7 whole cell and membrane extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GBA antibody [C1C3] (GTX101267) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GBA antibody [C1C3] (GTX101267) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](https://www.genetex.com/upload/website/prouct_img/normal/GTX101267/GTX101267_43817_20240920_WB_TPM_watermark_24092600_361.webp "Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GBA antibody [C1C3] (GTX101267) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.")
![GBA antibody [C1C3] detects GBA protein by immunofluorescent analysis. Sample: MCF-7 cells were fixed in ice-cold MeOH for 5 min. Green: GBA stained by GBA antibody [C1C3] (GTX101267) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920).](https://www.genetex.com/upload/website/prouct_img/normal/GTX101267/GTX101267_45098_20250620_ICC_IF_25070323_101.webp "GBA antibody [C1C3] detects GBA protein by immunofluorescent analysis. Sample: MCF-7 cells were fixed in ice-cold MeOH for 5 min. Green: GBA stained by GBA antibody [C1C3] (GTX101267) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920).")
Wild-type (WT) and GBA knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GBA antibody [C1C3] (GTX101267) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GBA antibody [C1C3]
GTX101267
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetGBA1
Overview
- SupplierGeneTex
- Product NameGBA antibody [C1C3]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1.32 mg/ml
- ConjugateUnconjugated
- Gene ID2629
- Target nameGBA1
- Target descriptionglucosylceramidase beta 1
- Target synonymsGBA, GCB, GLUC, lysosomal acid glucosylceramidase, D-glucosyl-N-acylsphingosine glucohydrolase, SGTase, acid beta-glucosidase, alglucerase, beta-GC, beta-glucocerebrosidase, beta-glucosylceramidase 1, cholesterol glucosyltransferase, cholesteryl-beta-glucosidase, glucocerebrosidase, glucosidase, beta, acid, glucosylceramidase-like protein, imiglucerase, lysosomal acid GCase, lysosomal cholesterol glycosyltransferase, lysosomal galactosylceramidase, lysosomal glucocerebrosidase, lysosomal glycosylceramidase
- HostRabbit
- IsotypeIgG
- Protein IDP04062
- Protein NameLysosomal acid glucosylceramidase
- Scientific DescriptionThis gene encodes a lysosomal membrane protein that cleaves the beta-glucosidic linkage of glycosylceramide, an intermediate in glycolipid metabolism. Mutations in this gene cause Gaucher disease, a lysosomal storage disease characterized by an accumulation of glucocerebrosides. A related pseudogene is approximately 12 kb downstream of this gene on chromosome 1. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq]
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
Datasheet
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