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GBA3 / CBG Antibody

LS-C409376
LifeSpan BioSciences
ApplicationsImmunoFluorescence, Western Blot
Product group Antibodies
TargetGBA3
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    GBA3 / CBG Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 18kDa/53kDa, while the observed MW by Western blot was 54kDa.
  • Applications
    ImmunoFluorescence, Western Blot
  • Applications Supplier
    IF (1:50 - 1:100), WB (1:500 - 1:2000) The predicted MW is 18kDa/53kDa, while the observed MW by Western blot was 54kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID57733
  • Target name
    GBA3
  • Target description
    glucosylceramidase beta 3 (gene/pseudogene)
  • Target synonyms
    CBG; CBGL1; cytosolic beta-glucosidase; cytosolic beta-glucosidase-like protein 1; cytosolic GCase; cytosolic glycosylceramidase; GLUC; glucosidase beta acid 3; glucosidase, beta, acid 3 (cytosolic); klotho-related protein; KLRP
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of GBA3 using anti-GBA3 antibody (A08549-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: rat kidney tissue lysates, Lane 3: mouse liver tissue lysates, Lane 4: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GBA3 antigen affinity purified polyclonal antibody (Catalog # A08549-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GBA3 at approximately 50 kDa. The expected band size for GBA3 is at 54 kDa.
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