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ICC/IF analysis of COS7 cells transiently transfected with GBE1 plasmid using GTX84452 GBE1 antibody [1D11].
ICC/IF analysis of COS7 cells transiently transfected with GBE1 plasmid using GTX84452 GBE1 antibody [1D11].
ICC/IF analysis of COS7 cells transiently transfected with GBE1 plasmid using GTX84452 GBE1 antibody [1D11].

GBE1 antibody [1D11]

GTX84452
GeneTex
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetGBE1
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Overview

  • Supplier
    GeneTex
  • Product Name
    GBE1 antibody [1D11] - Overexpression Validated
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:2000. ICC/IF: 1:50-1:100. IHC-P: 1:50. FACS: 1:100. IP: 2ug/500ul. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Flow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    1D11
  • Concentration
    1.1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID2632
  • Target name
    GBE1
  • Target description
    1,4-alpha-glucan branching enzyme 1
  • Target synonyms
    1,4-alpha-glucan-branching enzyme; amylo-(1,4 to 1,6) transglucosidase; amylo-(1,4 to 1,6) transglycosylase; APBD; brancher enzyme; GBE; glucan (1,4-alpha-), branching enzyme 1; glycogen branching enzyme; GSD4
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ04446
  • Protein Name
    1,4-alpha-glucan-branching enzyme
  • Scientific Description
    Required for sufficient glycogen accumulation. The alpha 1-6 branches of glycogen play an important role in increasing the solubility of the molecule and, consequently, in reducing the osmotic pressure within cells.
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of GBE1 using anti-GBE1 antibody (A04694-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates, Lane 2: human U251 whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: rat liver tissue lysates, Lane 5: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GBE1 antigen affinity purified polyclonal antibody (Catalog # A04694-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GBE1 at approximately 80 kDa. The expected band size for GBE1 is at 80 kDa.
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