![WB analysis of various cell lines using GTX84452 GBE1 antibody [1D11]. Loading : 35 ug per lane WB analysis of various cell lines using GTX84452 GBE1 antibody [1D11]. Loading : 35 ug per lane](https://www.genetex.com/upload/website/prouct_img/normal/GTX84452/GTX84452_4374_WB_w_23061420_360.webp)
WB analysis of various cell lines using GTX84452 GBE1 antibody [1D11]. Loading : 35 ug per lane
GBE1 antibody [1D11]
GTX84452
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetGBE1
Overview
- SupplierGeneTex
- Product NameGBE1 antibody [1D11]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:2000. ICC/IF: 1:50-1:100. FCM: 1:100. IP: 2ug/500ul. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID1D11
- Concentration1.1 mg/ml
- ConjugateUnconjugated
- Gene ID2632
- Target nameGBE1
- Target description1,4-alpha-glucan branching enzyme 1
- Target synonymsAPBD, GBE, GSD4, 1,4-alpha-glucan-branching enzyme, amylo-(1,4 to 1,6) transglucosidase, amylo-(1,4 to 1,6) transglycosylase, brancher enzyme, glucan (1,4-alpha-), branching enzyme 1, glycogen branching enzyme
- HostMouse
- IsotypeIgG1
- Protein IDQ04446
- Protein Name1,4-alpha-glucan-branching enzyme
- Scientific DescriptionRequired for sufficient glycogen accumulation. The alpha 1-6 branches of glycogen play an important role in increasing the solubility of the molecule and, consequently, in reducing the osmotic pressure within cells.
- ReactivityHuman, Mouse
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC41116161
![FACS analysis of Jurkat cells using GTX84452 GBE1 antibody [1D11]. Red : Primary antibody Blue : Negative control antibody FACS analysis of Jurkat cells using GTX84452 GBE1 antibody [1D11]. Red : Primary antibody Blue : Negative control antibody](https://www.genetex.com/upload/website/prouct_img/normal/GTX84452/GTX84452_419_FACS_w_23061420_104.webp)
![ICC/IF analysis of COS7 cells transiently transfected with GBE1 plasmid using GTX84452 GBE1 antibody [1D11]. ICC/IF analysis of COS7 cells transiently transfected with GBE1 plasmid using GTX84452 GBE1 antibody [1D11].](https://www.genetex.com/upload/website/prouct_img/normal/GTX84452/GTX84452_1084_ICCIF_w_23061420_749.webp)
![WB analysis of human tissues (Lane 1-Testis ; Lane 2-Omentum ; Lane 3-Uterus ; Lane 4-Breast ; Lane 5-Brain ; Lane 6-Liver ; Lane 7-Ovary ; Lane 8-Thyroid gland ; Lane 9-colon ; Lane 10-spleen) using GTX84452 GBE1 antibody [1D11]. Loading : 10 ug per lane Dilution : 1:500 WB analysis of human tissues (Lane 1-Testis ; Lane 2-Omentum ; Lane 3-Uterus ; Lane 4-Breast ; Lane 5-Brain ; Lane 6-Liver ; Lane 7-Ovary ; Lane 8-Thyroid gland ; Lane 9-colon ; Lane 10-spleen) using GTX84452 GBE1 antibody [1D11]. Loading : 10 ug per lane Dilution : 1:500](https://www.genetex.com/upload/website/prouct_img/normal/GTX84452/GTX84452_4494_WB_w_23061420_170.webp)
![ICC/IF analysis of HepG2 cells using GTX84452 GBE1 antibody [1D11]. ICC/IF analysis of HepG2 cells using GTX84452 GBE1 antibody [1D11].](https://www.genetex.com/upload/website/prouct_img/normal/GTX84452/GTX84452_1085_ICCIF_w_23061420_133.webp)
![FACS analysis of HEK293T cells transfected with either GBE1 plasmid(Red) or empty vector control plasmid(Blue) using GTX84452 GBE1 antibody [1D11]. FACS analysis of HEK293T cells transfected with either GBE1 plasmid(Red) or empty vector control plasmid(Blue) using GTX84452 GBE1 antibody [1D11].](https://www.genetex.com/upload/website/prouct_img/normal/GTX84452/GTX84452_420_FACS_w_23061420_103.webp)
![IP analysis of DDDDK tagged GBE1 overexpressed HEK293T lysate using GTX84452 GBE1 antibody [1D11].After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDDDK polyclonal antibody. IP reaction : 2μg antibody / 500μl cell lysate IP analysis of DDDDK tagged GBE1 overexpressed HEK293T lysate using GTX84452 GBE1 antibody [1D11].After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDDDK polyclonal antibody. IP reaction : 2μg antibody / 500μl cell lysate](https://www.genetex.com/upload/website/prouct_img/normal/GTX84452/GTX84452_3353_IP_w_23061420_322.webp)
![WB analysis of HEK293T cells transfected with GBE1 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84452 GBE1 antibody [1D11]. Loading : 5 ug per lane WB analysis of HEK293T cells transfected with GBE1 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84452 GBE1 antibody [1D11]. Loading : 5 ug per lane](https://www.genetex.com/upload/website/prouct_img/normal/GTX84452/GTX84452_4373_WB_w_23061420_358.webp)





![IP analysis of DDDDK tagged GBE1 overexpressed HEK293T lysate using GTX84453 GBE1 antibody [4D9].After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDDDK polyclonal antibody. IP reaction : 2μg antibody / 500μl cell lysate](https://www.genetex.com/upload/website/prouct_img/normal/GTX84453/GTX84453_3354_IP_w_23061420_693.webp)
![IHC-P analysis of human ovary tissue using GTX84454 GBE1 antibody [3C8]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.](https://www.genetex.com/upload/website/prouct_img/normal/GTX84454/GTX84454_2740_IHC-P_w_23061420_212.webp)