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ProductsBack/GBE1 Mouse Monoclonal Antibody [Clone ID: OTI5B7]
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GBE1 (RC204152, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GBE1. Positive lysates LY400056 (100ug) and LC400056 (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GBE1 (RC204152, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GBE1. Positive lysates LY400056 (100ug) and LC400056 (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GBE1 (RC204152, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GBE1. Positive lysates LY400056 (100ug) and LC400056 (20ug) can be purchased separately from OriGene.

GBE1 Mouse Monoclonal Antibody [Clone ID: OTI5B7]

CF500802
OriGene
ApplicationsFlow Cytometry, ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetGBE1
Price on request
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    GBE1 Mouse Monoclonal Antibody [Clone ID: OTI5B7]
  • Delivery Days Customer
    14
  • Applications
    Flow Cytometry, ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI5B7
  • Gene ID2632
  • Target name
    GBE1
  • Target description
    1,4-alpha-glucan branching enzyme 1
  • Target synonyms
    APBD, GBE, GSD4, 1,4-alpha-glucan-branching enzyme, amylo-(1,4 to 1,6) transglucosidase, amylo-(1,4 to 1,6) transglycosylase, brancher enzyme, glucan (1,4-alpha-), branching enzyme 1, glycogen branching enzyme
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ04446
  • Protein Name
    1,4-alpha-glucan-branching enzyme
  • Scientific Description
    Carrier-free (BSA/glycerol-free) GBE1 mouse monoclonal antibody, clone OTI5B7 (formerly 5B7)
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

MSDS

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FAQ

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Figure 1. Western blot analysis of GBE1 using anti-GBE1 antibody (A04694-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates, Lane 2: human U251 whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: rat liver tissue lysates, Lane 5: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GBE1 antigen affinity purified polyclonal antibody (Catalog # A04694-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GBE1 at approximately 80 kDa. The expected band size for GBE1 is at 80 kDa.
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