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ProductsBack/GBL Antibody - middle region (OAAB17572)

GBL Antibody - middle region (OAAB17572)

OAAB17572
Aviva Systems Biology
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
TargetMLST8
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Overview

  • Supplier
    Aviva Systems Biology
  • Product Name
    GBL Antibody - middle region (OAAB17572)
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Format
    Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
  • Gene ID64223
  • Target name
    MLST8
  • Target description
    MTOR associated protein, LST8 homolog
  • Target synonyms
    gable; GbetaL; GBL; LST8; mammalian lethal with SEC13 protein 8; POP3; protein GbetaL; target of rapamycin complex subunit LST8; TORC subunit LST8; WAT1
  • Host
    Rabbit
  • Storage Instruction
    2°C to 8°C,-20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MLST8 using anti-MLST8 antibody (A05357-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: rat kidney tissue lysates, Lane 3: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MLST8 antigen affinity purified polyclonal antibody (Catalog # A05357-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MLST8 at approximately 36 kDa. The expected band size for MLST8 is at 36 kDa.
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