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GCC1 antibody [C1C3]

GTX120148
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetGCC1
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Overview

  • Supplier
    GeneTex
  • Product Name
    GCC1 antibody [C1C3]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.96 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID79571
  • Target name
    GCC1
  • Target description
    GRIP and coiled-coil domain containing 1
  • Target synonyms
    GCC1P, GCC88, GRIP and coiled-coil domain-containing protein 1, golgi coiled-coil 1, golgi coiled-coil protein 1, peripheral membrane Golgi protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96CN9
  • Protein Name
    GRIP and coiled-coil domain-containing protein 1
  • Scientific Description
    The protein encoded by this gene is a peripheral membrane protein. It is sensitive to brefeldin A. This encoded protein contains a GRIP domain which is thought to be used in targeting. It may play a role in the organization of trans-Golgi network subcompartment involved with membrane transport. [provided by RefSeq]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Bhatt JM, Hancock W, Meissner JM, et al. Promiscuity of the catalytic Sec7 domain within the guanine nucleotide exchange factor GBF1 in ARF activation, Golgi homeostasis, and effector recruitment. Mol Biol Cell. 2019,30(12):1523-1535. doi: 10.1091/mbc.E18-11-0711
    Read this paper

Datasheet

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Figure 1. Western blot analysis of GCC1 using anti-GCC1 antibody (A12100-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse Neuro-2a whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human U-87MG whole cell lysates, Lane 4: rat ovary tissue lysates, Lane 5: rat liver tissue lysates, Lane 6: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GCC1 antigen affinity purified polyclonal antibody (Catalog # A12100-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GCC1 at approximately 100 kDa. The expected band size for GCC1 is at 100 kDa.
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